Effect of Shikoni on Phosphomonoesterase Activity of Mycobaterium Tuberculosis Protein Tyrosine Phosphotases (PTPs)
Keywords:
Shikoni, Phosphatases activity, Mycobacterium Tuberculosis, Protein Tyrosine Phosphatases (PTPs)Abstract
Several factors such as patient noncompliance and drug resistance affect the success rate of conventional treatments against tuberculosis and other pathogenic diseases; therefore there is need to improve the available therapeutic ways used in targeting tuberculosis treatment as well as design of new drugs that could act more rapidly on them. This research aimed to determine the effect of shikoni on phosphomonoesterase activity of mycobacterium tuberculosis using protein tyrosine phosphatases as enzyme inhibitor. Purified homogenous PTPs were obtained and shikoni phosphomonoesterase activity was determined by measuring the p-nitrophenol releases from the pNPP substrate. PTP acts as potential drug target which enables strategies to develop modern treatment regimen and regulate the levels of protein tyrosine phosphorylation under normal and pathological conditions.
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